Our findings raise another interesting issue relating to plasmid accumulation and life span
The results show that replication of 2 micron DNA occurs by a semiconservative mechanism where each of the plasmid molecules replicates once each
In this study, we characterized the major attributes of the 2-micron and centromeric plasmids typically used in yeast by examining the impact of choice of
The two strands of mtDNA differ in their base composition, with one
The elucidation of the structure of Thus, it requires a yeast origin of replication
The 2 micron plasmid is a relatively small double stranded circular DNA genome (~6
The organization of the 2-micron plasmid found in laboratory strains of S
Each end of the bubble is a replication fork, a Y-shaped junction where double-stranded DNA is separated into two single strands
6 x 10 6 nucleotide pairs
Part # 1
Which is a key concept first discovered about viruses that distinguished them from other microorganisms? A
They were small enough to pass through a
In order to develop a procedure for transformation of the industrial yeast Torulaspora delbrueckii, we have constructed a set of recombinant plasmids carrying Saccharomyces cerevisiae ARS and 2 microns origin of replication and kanamycin-G418 resistance gene of Tn903(601) as a selective marker
Our results indicate that a mechanism exists which insures that the origin of replication of each 2 micron DNA molecule is activated each S phase
Store the plasmid at -20 ℃
Replication origins in the yeast Saccharomyces cerevisiae are identified as autonomous replication sequence (ARS) elements
♦ pUC plasmid origin of replication and the ampicillin-resistance gene (bla), which allow for replication and selection in E
Origins of replication and copy numbers of various plasmids and cosmids
Johnson & Johnson
cerevisiae vectors are shuttle vectors